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J Neurooncol. 2013 Nov;115（2）:179-88. doi: 10.1007/s11060-013-1223-2. Epub 2013 Aug 13. MiR-29c inhibits glioma cell proliferation, migration, invasion and angiogenesis. Fan YC1, Mei PJ, Chen C, Miao FA, Zhang H, Li ZL. Author information 1Department of Neurosurgery, The Affiliated Hospital of Xuzhou Medical College, 99 West Huai-hai Road, Xuzhou, 221002, Jiangsu, China, fanyuechaoxyfy@163.com.
Abstract Previous studies reported that miR-29c is significantly downregulated in several tumors. However, little is known about the effect and molecular mechanisms of action of miR-29c in human glioma. Using quantitative RT-PCR, we demonstrated that miR-29c was significantly downregulated in glioma cell lines and human primary glioma tissues, compared to normal human astrocytes and matched non-tumor associated tissues （P < 0.05, χ（2） test）. Overexpression of miR-29c dramatically reduced the proliferation and caused cessation of cell cycle. The reduced cell proliferation is due to G1 phase arrest as cyclin D1 and cyclin E are diminished whereas p27 and p21 are upregulated. We further demonstrated that miR-29c overexpression suppressed the glioma cell migration and invasion abilities by targeting MMP-2. In addition, we also found that overexpression of miR-29c sharply inhibited angiogenesis, which correlated with down-regulation of VEGF. The data indicate that miR-29c may be a tumor suppressor involved in the progression of glioma. PMID: 23943502 DOI: 10.1007/s11060-013-1223-2
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